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KMID : 0378019740170081085
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New Medical Journal
1974 Volume.17 No. 8 p.1085 ~ p.1094
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Studies on the Circadian Activity of Liver Cell of One Day Post-Natal Male Chick
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Abstract
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In order to estimate the circadian variation of cell cycle of liver cell 3H-thymidine was introduced into one day postnatal male chicks of Shaver species and autoradiography was carried out.
The chickss were divided into two experimental groups; AM and PM groups.
At the beginning of every hour, starting from 5:03 AM for 25 hours, 3 healthy chicks were carefully selected and injected with given doses of (3)^H-thymidine to be used as the AM group. PM chickss were treated in the same manner starting from 2:00 PM.
AM chicks were sacrificed at 6:00 AM and PM chicks at 3:03 PM next day. The livers of these animals were autoradiographed.
These results were analyzed and compared with previous studies.
The results were summarized as follows:
1. The duration of TG was 398 hours in AM chicks and 530 hours in PM chicks.
2. The durarion of S phase was 17.5 hours in AM chicks and 10.6 hours in PM chicks.
3. The duration of G2 phase was 2.5 hours in AM chicks and 2.8 hours in PM chicks.
4. The duration of M phase was 1.0 hour in AM chicks and 1.2 hours in PM chicks.
5. The duration of Gl phase was 377 hours in AM chicks and 516 hours in PM chicks.
Labelling indices of AM and PM chicks were 4.4% and 2.0% respectively.
Difference between the generation times of AM and PM chicks was significant showing the influence of circadian rhythm.
Difference between the durations of S phases of AM and PM chicks was significant.
Durations of G, phases of AM and PM groups revealed a remarkable difference.
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